Asia-Pacific Journal of Pharmacotherapy & Toxicology

Research Article | Open Access

Phytochemical Screening and biological potentials of Musa × paradisiaca L. leaves extract as anticancer and antioxidant agents

Aakash Deep1, Priya1, Om Narayan Upadhyay1, Sanjiv Kumar1, Harish Kumar1, Nitin Bansal1, Arun Kumar Sharma2, Deepak Wadhwa3 and Surender Kumar3

1. Department of Pharmaceutical Sciences, Chaudhary Bansi Lal University, Bhiwani, Haryana, India.

2. Department of Pharmacology, Amity Institute of Pharmacy, Amity University, Gurugram, Haryana, India.

3. Department of Chemistry, Chaudhary Bansi Lal University, Bhiwani, Haryana, India.

Correspondence: Aakash Deep (Department of Pharmaceutical Sciences, Chaudhary Bansi Lal University, Bhiwani, Haryana, India-127021; E-mail: aakashdeep82@gmail.com).

Asia-Pacific Journal of Pharmacotherapy & Toxicology 2021, 1(1): 19-27. https://doi.org/10.32948/ajpt.2021.12.10

Received: 01 Jul 2021 | Accepted: 18 Sep 2021 | Published online: 12 Dec 2021

Abstract

Background Many of these plants, have therapeutic effects and can be extracted and used in preparation of drugs, used directly or in combination with other plant extracts for medication which is a common practice in developing counties. Unfortunately, many of those who utilize these plants therapeutically do not have adequate knowledge or training in the safe use of the products. For these reasons, natural plant products need to be standardized and preliminary studies done to evaluate possible risks such as undesirable side effects, overdose and toxicity.
Results Ethyl acetate extract of Musa × paradisiaca L shown anticervical carcinoma and anti-malignant melanoma activity in our study. Antioxidant activity demonstrated, that Musa × Paradisiaca L. leaves ethyl extract exhibited % inhibition at absorbance 517 nm with IC50 values = 3.70 to 45.50 at different concentration and compared with ascorbic acid as standard drug.
Conclusions The present study indicates the anticancer and antioxidant activity on the basis of biological and phytochemical screening of Musa × paradisiaca L leaves extract. Ethyl acetate extract of leaves was evaluated for its anticancer activity. In vitro anticancer activity of extract were estimated by measuring significant inhibition of HeLa and A375 cell lines  by MTT assay. The MTT assay clearly indicates that the inhibition or inhibitory activity of the extract was concentration dependent. Maximum inhibition of cell growth was found at the concentration of 320 µg/ml which was 54.35 and 55.97, respectively for HeLa and A375 cell lines. Therefore, 320 µg/ml concentration of extract was used to study the IC50 value that was calculated as 249.1 and 224.4, respectively. Antioxidant activity demonstrated that, plant extract exhibited percentage inhibition with IC50 values = 3.70 to 45.50 at different concentration and compared with ascorbic acid as standard drug.

Key words Musa × paradisiaca L plant, phytochemical screening, anticancer activity, antioxidant activity, MTT assay